![]() Next-generation sequencing Polymerase chain reaction Urinary tract infection Urine culture. Llusern’s first test kit is a panel of urinary tract infection (UTI) assays including E-coli, Klebsiella and Staphylococcus spp. We found good evidence that molecular methods detect more bacteria than culture however, the clinical implications to support their routine use are unclear. Jant Pharmacal Corporation, founded in 1986, develops and markets medical devices, including complementary and companion point-of-care diagnostics. UK diagnostics manufacturer, Llusern Scientific, will preview its Lodestar DX molecular diagnostic platform at the upcoming Medica 2022 in Dusseldorf.Llusern will be part of the Wales Government pavilion in Hall 16, stand 38. We compare culture-independent molecular methods with urine culture in the management of urinary tract infection. Additional data comparing patient symptoms and cure rates following antibiotic selection directed by molecular methods compared with culture are needed to elucidate their place in UTI care. Moderately strong evidence exists that molecular diagnostics demonstrate increased sensitivity in detecting urinary bacteria at the expense of poor specificity in controls. ![]() There is insufficient evidence to compare patient symptomatic responses to antibiotic therapy guided by molecular testing versus standard susceptibility testing. Phenotypic and genotypic resistomes are concordant in approximately 85% of cases. PCR protocols designed to detect a diverse range of microbes had increased sensitivity and species diversity compared with culture. A meta-analysis of seven similar studies utilizing NGS demonstrates that NGS is more sensitive in the identification of urinary bacteria and detects greater species diversity per urine sample than culture. Included reports compared NGS (nine studies) and PCR (six studies) to urine culture. Fifteen publications were selected for inclusion. ‘Multi-Center Clinical Trial Using a Novel Semi-Quantitative Multiplex-PCR Based Diagnostic Panel for Urinary Tract Specimens. Risk of bias and methodological quality were assessed using the Cochrane risk of bias tool and Newcastle Ottawa Scale. Studies involving pregnant women, ureteral stones, ureteral stents, and percutaneous nephrostomy tubes were excluded. ![]() We performed a critical review of Embase, Ovid, and PubMed in February 2022 according to the Preferred Reporting Items for Systematic Review and Meta-analyses statement. To systematically evaluate the evidence comparing the diagnostic and therapeutic values of molecular diagnostic methods to urine culture in the management of UTI in adults. Next-generation sequencing (NGS) and polymerase chain reaction (PCR) are culture-independent molecular methods available for commercial use to diagnose UTI. Urine culture has low sensitivity in the diagnosis of urinary tract infection (UTI). ![]()
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